Our objective was to develop and evaluate Ga-68-and Cu-64-labeled albumin conjugates for PET imaging of sentinel lymph nodes. Four different albumin conjugates were prepared starting from NOTA-HSA. The lymph node uptake of Ga-68-and Cu-64-labeled albumin conjugates was investigated after subcutaneous injection into the foot pad of Wistar rats. A pig model was utilized for further biological evaluation of the lymph node uptake. For all the four conjugates, radiolabeling with Ga-68 and Cu-64 resulted in> 95% radiochemical yield. Denatured and mannosylated Ga-68 NOTA-HSA revealed the highest popliteal lymph node uptake in rats (2.78%±0.38% IDand 6.13%±1.13% ID 10 min and 60 min pi, resp.). Thepopliteal lymph node reached its maximumactivity after approximately 120 min and remained constant for denatured and mannosylated Cu-64 NOTA-HSA at least up to 240 min pi In a pig model, 2% of the injected dose of this compound was found in the sentinel lymph node 60 min after subcutaneous injection. In conclusion, PET imaging of sentinel lymph nodes with Ga-68-and Cu-64-labeled denatured NOTA-Man-HSA could be successfully demonstrated and deserves further investigations.
1 Jan 2013
Volume: 42 Issue: 1 Pages: S149
European Biophysics Journal