This work reports light scattering measurements on DNA in aqueous solutions (100 mM NaCl, 1 mM EDTA and 10 mM Tris–HCl buffer, pH 7.8) over a wide range of molecular weights (102–105 base pairs) and shows that, in the above standard solvent, shorter chains (104 base pairs) behave in a different manner. Dynamic and static light scattering and SEM analysis indicate that DNA molecules 105 base pairs long, condense into compact structures in our solvent conditions. Calculations done using a wormlike model are also presented and discussed in comparison both to our experimental data and to other data reported in the literature.
1 Oct 2009
Volume: 45 Issue: 3 Pages: 242-247
International journal of biological macromolecules